anti f4 80 (Proteintech)

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Proteintech anti f4 80
Anti F4 80, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 739 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+f4+80/F4%2F80+Antibody/pmc12972984-356-51-74
Average 96 stars, based on 739 article reviews

anti f4 80 - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Orf Virus Remodels Tumor Immune Microenvironment in Melanoma Mouse Models Revealed by Single-Cell RNA Sequencing
Article Snippet: .. A mixture of primary antibodies—antiTREM2 (Proteintech, China, 68723-1-Ig) and anti-F4/80 (Proteintech, China, 28463-1-AP)—diluted 1:400 was applied, and sections were incubated overnight at 4°C. .. After three PBST washes (each for 3 minutes), sections were incubated with a mixture of Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Abcam, UK, ab150080), Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Abcam, UK, ab150113) at 37°C for 1 hour, followed by another round of PBST washes.

Article Title: Cuproptosis promotes inflammatory osteolysis via GYS1-mediated glycogen metabolism
Article Snippet: The antibodies as follows: H3K27me3 (Abcam, ab6002), DLAT (Proteintech, 68303-1-lg). .. Sections were deparaffinized and rehydrated, and then incubated in 3% H 2 O 2 for 10 min, boiled with citrate buffer (pH 6.0) for antigen retrieval, and then blocked with 5% serum followed by incubating overnight at 4 °C with primary antibody, including anti-F4/80 (Proteintech, 28463). .. Following washing, secondary antibodies and streptavidin-biotin complex (SABC) were applied with SABC-POD kits (BOSTER, SA1022).

Article Title: IRF6 induces endothelial dysfunction through the transcriptional activation of NDRG1 and aggravates low shear stress-mediated atherosclerosis
Article Snippet: Cell samples were permeabilized with 0.1% Triton X-100 (ST795, Beyotime, China) for 30 min after fixation with 4% paraformaldehyde. .. Then, tissue or cell sections were blocked with 1% BSA (A602440-0050, Sangon, China) for 15 min and then incubated with anti-CD31 (1:50, Santa Cruz Biotechnology Cat# sc-376764, RRID: AB_2801330 ), anti-8-OHdG (1:100, Santa Cruz Biotechnology Cat# sc-393871, RRID: AB_2892631 ), anti-eNOS (1:100, Affinity Biosciences Cat# AF0096, RRID: AB_2833277 ), anti-F4/80 (1:100, Proteintech Cat# 28463-1-AP, RRID: AB_2881149 ), and anti-VCAM1 (1:100, ABclonal Cat# A19131, RRID: AB_2862624 ) primary antibodies overnight. .. After that, sections were incubated with the corresponding fluorescent-coupled secondary antibody, goat anti-mouse IgG (1:200, Abcam Cat# ab97035, RRID: AB_10680176 ) or goat anti-rabbit IgG (1:200, Abcam Cat# ab6939, RRID: AB_955021 ) for 1 h at room temperature, and then nuclei were stained with DAPI (D106471, Aladdin, China).

Immunohistochemical staining:

Article Title: NMN protects cisplatin-associated AKI via NAD + /SIRT1 pathway
Article Snippet: .. Immunohistochemical staining was performed by Friss Biotechnology (China) using the following primary antibodies: anti-SIRT1 (1:250, catalog number 60303-1-Ig; Proteintech, China), anti-Ly6G (1:200, catalog number 24633-1-AP; Proteintech, China), anti-NGAL (1:1000, catalog number ab216462; Abcam, UK), anti-F4/80 (1:2000, catalog number 28463-1-AP; Proteintech, China), and anti-KIM-1 (1:200, catalog number PA5-98302; Proteintech, China). ..

Staining:

Article Title: Tenascin C + myofibroblasts exacerbate vascular neointimal hyperplasia by propagation of nerve-macrophage interactions in mice.
Article Snippet: After incubating Mu/Rb-HRP secondary antibody (AiFang biological, AFIHC001) for 1 h at RT, developed by staining with DAB kit (Servicebio, G1212) for 1min. .. The sections were blocked with blocking buffer (5% BSA and 0.2% Triton X-100 in PBS) for 1 h and stained with anti-TNC (1:200, Proteintech, 67710-1-Ig), anti-Ki67 (1:200, Servicebio, GB121141), anti-αSMA (1:500, Cell Signaling Technology, 48938S), anti-Fn1 (1:200, Proteintech, 66042-1-Ig), anti-Postn (1:200, Proteintech, 66491-1-Ig), antiCD68 (1:200, BIO-RAD, MCA1957), anti-CD11c (1:200, Cell Signaling Technology, 97585), anti-F4/80 (1:200, Proteintech, 29414-1-AP), antiPostn (1:200, Proteintech, 66491-1-Ig), anti-Synapsin1/2 (1:100, SYSY, 106 002), anti-TNF (1:200, Proteintech, 17590-1-AP), anti-NOS2 (1:200, Invitrogen, PA1-036), anti-ARG1(1:200, Cell Signaling Technology, 93668), anti-CCL2(1:200, Proteintech, 26161-1-AP) anti-CD45 (1:200, Abcam, ab10558) and anti-βIII-tubulin (1:1000, Abcam, ab78078) primary antibodies overnight at 4 °C. .. Afterwards, the sectionswere rinsed three times in PBS and stained with corresponding secondary antibodies (all from Invitrogen) for 1 h at RT.

Blocking Assay:

other:

Article Title: Serum metabolic fingerprinting for diagnosis and therapeutic applications of ovarian endometriosis
Article Snippet: Anti-F4/80 , Proteintech , Cat# 28463-1-AP, RRID: AB_2881149.

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